扬州大学学报（农业与生命科学版）2012，Vol.33Issue(3)：1-4,4.
高致病性毒力岛Irp1介导的细菌对Vero细胞黏附作用
The adhesion effect with Vero cells mediated by HPI-Irp1
摘要
Abstract
High pathogenicity island (HP1) is first detected in Yer.sinia. It was found in many kinds of Escherichia coli. The irp1 gene and fyuA gene were analysed by DNAStar to look for the surface probability plot. Eight fragments of irp1 gene and two of fyuA gene were selected. Primers were designed to amplify the ten fragments. The PCR products were cloned into expressive vector pET32a and transformed into fimbriae negative host bacteria BL21. The recombinant bacteria were induced with IPTG to expression and mixed with the single layer Vero cells growing well in dishes. After washed six times with Hank's solution, the cells were observed with oil microscope. The results showed that the recombinant bacteria BL21-rpET32a-irp1-1 including irp1-1 fragment had strong reaction with Vero cells. The production irp1-1 gene encoded may act as an bridge between the bacteria and host cells during process of pathogenicity.关键词
大肠杆菌/高致病性毒力岛(HPI)/Irp1/黏附作用Key words
Escherichia coli / high pathogenicity island (HP1)/ Irp1/ adhesion effect分类
农业科技引用本文复制引用
金文杰,郑志明,吉荣,钱文正,秦爱建..高致病性毒力岛Irp1介导的细菌对Vero细胞黏附作用[J].扬州大学学报（农业与生命科学版）,2012,33(3):1-4,4.基金项目
国家自然科学基金资助项目(30800822) （30800822）
扬州大学创新基金(2011CXJ067) （2011CXJ067）
江苏省青蓝工程计划项目(2010-11) （2010-11）
教育部创新团队项目(PCSIRT) （PCSIRT）
江苏省优势学科项目(PAPD) （PAPD）
