扬州大学学报（农业与生命科学版）2012，Vol.33Issue(3)：32-39,8.
鳡胰岛素样生长因子1基因的克隆及其组织特异性表达分析
Cloning and expression analysis of the insulin-like growth factor 1 in Elopichthys bambusa
摘要
Abstract
The objective of the present research was to clone full-length IGFl cDNA sequence and to determine its expression in various tissues from Elopichthys bambusa. Reverse transcription PCR (RT-PCR), rapid-amplification of cDNA ends (RACE) were used to amplify full-length of IGF1 cDNA. Semi-quantatative RT-PCR was used to compare IGF1 gene expression levels from various tissues. A full-length of IGF1 cDNA sequence in E. bambusa was obtained, which included a complete open reading frame (ORF) of 486 bp, a 5 -untranslated region of 218 bp, and a 3 -untranslated region of 140 bp. The putative peplide contained a signal sequence of 44 amino acid, a mature peptide of 70 amino acid in the functional domain- and an extended carboxyl-terminal peptide of 47 amino acid in domain E. IGFl from E. bambusa showed an amino acid identity of 94. 41%-99. 38% with that from Cy print formes. The phylogenetic analyses showed that IGF1 from E. bambusa had closest relationship with other fishes in Cypriniformes. Furthermore, IGF1 mRNA levels were highest in liver but relatively low or absent in heart. A part of IGF1 promoter sequence was cloned by using Genome Walking, which included a Myogenic Differentiation Antigen (Myo D). But there was not a consensus TATA box about 5' to the start site of transcription in IGF1. These results indicated the IGF1 promoter differed to the classic promoter. These findings provide clue for IGF1 involvement in the growth and development of E. bambusa and are potential basis for its effective breeding and propagation.关键词
鳡/胰岛素样生长因子1/启动子/开放性阅读框/表达Key words
Elopichthys bambusa/ IGF1, promoter/ ORF/ express分类
农业科技引用本文复制引用
郁建锋,李建林,徐建荣,卢祥云,柏洁,顾志良..鳡胰岛素样生长因子1基因的克隆及其组织特异性表达分析[J].扬州大学学报（农业与生命科学版）,2012,33(3):32-39,8.基金项目
国家自然科学基金资助项目(31072025) （31072025）
教育部出国留学回国人员科研启动基金资助项目(2011) （2011）
苏州市2010年度科技基础设施建设项目(SZSD201001) （SZSD201001）
农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放基金资助项目(KY2009047) （KY2009047）
