江西农业学报2012，Vol.24Issue(10)：1-3,8,4.
椰子CBF基因的克隆研究
Study on Cloning of CBF Gene from Coconut (Cocos nucifera)
摘要
Abstract
In order to elucidate the molecular mechanism of coconuts cold resistance, this study designed primers according to the CBF gene sequence of oil palm, and then amplified the genomic DNA of Cocos nucifera by using PCR method. The PCR products were retrieved, linked, transformed and sequenced; finally, a DNA sequence of Cocos nucifera was obtained. This sequence had the highest identity (99%) with CBF gene of oil palm, and it was confirmed to be CBF gene. Through the clustering analysis of the cloned CBF gene of coconut and CBF genes of graminaceous crops, the high homology was found between the cloned CBF gene and HvCBF1, Os-DREB1E, HvCBF11, TaCBF11.关键词
椰子/CBF基因/克隆/抗寒性Key words
Cocos nucifera/ CBF gene/ Cloning/ Cold resistance分类
农业科技引用本文复制引用
肖勇,杨耀东,曹红星,雷新涛,范海阔,赵松林,马子龙..椰子CBF基因的克隆研究[J].江西农业学报,2012,24(10):1-3,8,4.基金项目
中国热带农业科学院基本科研业务费专项(1630032012044). （1630032012044）
