江西农业学报2012，Vol.24Issue(10)：102-103,126,3.
钝顶螺旋藻藻蓝蛋白的提取及分子量研究
Study on Extraction of Phycocyanin from Spirulina platensis and Its Molecular Weight
张厚森 1尹利端 2赵厚民 1贾涛 1李新丽1
作者信息
- 1. 江苏省理化测试中心,江苏南京2100421
- 2. 烟台新时代健康产业有限公司,山东烟台264006
- 折叠
摘要
Abstract
The extraction of phycocyanin from Spirulina platensis was studied through the freeze - thaw method using the extraction ratio as index. Finally, the molecular weight of phycocyanin was studied through sodium dodecyl sulfate - polyacrylamkie gel elee-trophoresis (SDS - PAGE) and column chromatographic method. The results showed that the optimal technologic condition was as follows: solid -liquid ratio was 1: 22 (m/V) , temperature was 35 ℃, extract time was 2. 5 hours, and the extraction ratio of CPC was 3.19%. Its molecular weight was 40000 and 43200 Da, respectively with two different molecular weight testing methods.关键词
钝顶螺旋藻/藻蓝蛋白/提取/分子量Key words
Spirulina platensis/ Phycocyanin/ Extraction/ Molecular weight分类
农业科技引用本文复制引用
张厚森,尹利端,赵厚民,贾涛,李新丽..钝顶螺旋藻藻蓝蛋白的提取及分子量研究[J].江西农业学报,2012,24(10):102-103,126,3.
