江西农业学报2012，Vol.24Issue(10)：104-106,129,4.
肉制品中5种红曲色素的高效液相色谱测定
Determination of Five Monascus Pigments in Meat Products by High Performance Liquid Chromatography Method
摘要
Abstract
A high performance liquid chromatography (HPLC) method was set up to analyze the content of five Monascus pigments in meat products. The samples were extracted with methanol + ethyl acetate (2:8, v/v) , separated with Eclipse plus. C18 [5 m, 25 cm×4.6 mm (i. d.) ] analytical column, and gradient - eluted by using phosphoric acid solution ( pH =2.5±0.3) and aceto-nitrile as mobile phase with the flow rate of 1 mL/min. The five Monascus pigments were separated for 20 min and determined by HPLC with a diode array detector, and the determination wavelength was 400 nm. The correlation coefficients (r2) of this method were not less than 0. 99910, the limits of detecting the five Monascus pigments in meat products were between 0.52 mg/kg and 1. 05 mg/kg (S/N=3), and the average standard - adding recoveries ranged from 75% to 108% with RSDs (n=8) of 1.6% ~7.6% for all the compounds. The results indicated that this method was accurate, sensitive, highly reproducible and fast, and it was suitable for the a-nalysis of Monascus pigments in meat products.关键词
高效液相色谱(HPLC)/肉制品/红曲色素Key words
High performance liquid chromatography/ Meat product/ Monascus pigment分类
化学化工引用本文复制引用
胡丽芳,罗林广,李瑞丽,李伟红..肉制品中5种红曲色素的高效液相色谱测定[J].江西农业学报,2012,24(10):104-106,129,4.基金项目
农业部行业标准基金资助项目(农财发[2009]105号文). （农财发[2009]105号文）
