昆明医学院学报2012，Vol.33Issue(8)：21-26,6.
ICI118,551诱导胰腺癌细胞凋亡及其机制的实验研究
The Apoptosis of Human Pancreatic Cancer induced by ICI118,551 and Its Mechanism of Action
摘要
Abstract
Objective To investigate the effect of ICI118, 551 on pancreatic cancer cell apoptosis and its action mechanism. Methods The apoptotic index was determined by the flow cytometry assay. The cell morphology of apoptosis was observed by electron microscope and Hoechst 33324 fluorescein stain. The expression of ERK, Akt, caspase-3, caspase-9, Bcl-2 and Bax was analysed by Western blot. Results ICI118, 551 significantly induced apoptosis compared with (β 1-adrenergic antagonist metoprolol in MIA PaCa-2 and BxPC-3 cell lines. ICI118, 551 affected the expression of ERK, Akt, caspase-3, caspase-9, Bcl-2 and Bax. Conclusion P 2 adrenoceptor antagonist can block related cell signal pathways, then inhibit the expression of pro-invasive and anti-apoptotic factors in the downstream, and finally induce the apoptosis of pancreatic cancer cell.关键词
β2受体阻滞剂ICI118,551/凋亡/G1/S期阻滞Key words
ICI118, 551/Apoptosis/G1/S phase arrest分类
医药卫生引用本文复制引用
徐军,彭波,穆维靖,王铮,张东,马清涌..ICI118,551诱导胰腺癌细胞凋亡及其机制的实验研究[J].昆明医学院学报,2012,33(8):21-26,6.基金项目
国家自然科学基金资助项目(81172195) （81172195）
