昆明医科大学学报2012，Vol.33Issue(9)：11-14,25,5.
大豆苷元对人乳牙牙髓干细胞增殖和成骨分化的影响
Effects of Daidzein on Proliferation and Osteogenic Differentiation of Stem Cells Derived from Human Exfoliated Deciduous Teeth
武斌 1孟秋菊 1谢春 1黄谦 2魏波 1陈敏莹1
作者信息
- 1. 宝安区龙华医院口腔医学中心,广东深圳518109
- 2. 广东医学院整形外科研究所,广东湛江524000
- 折叠
摘要
Abstract
Objective To study the effects of daidzein on proliferation and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth ( SHED). Methods The third passage SHEDs were incubated with 0.1, 1, 10, 100 |xM daidzein, then cells proliferation curves were examined by MTT method. Alkaline phosphatase (ALP) , osteocalcin (OCN) and TGF- (3 1 were detected by alkaline phosphatase kit, radio-immunity method and TGF- f$ 1 Elisa kit, respectively. Results 0.1 (xM daidzein improved proliferation of SHED, 100 (xM daidzein, however, showed a depressant effect on proliferation. ALP, OCN and TGF-|5 1 expression were enhanced by daidzein in a dose-dependent manner. Conclusion Daidzein enhances proliferation and osteogenic differentiation of SHED, and the mechanism may be related with upregulating TGF?{$ 1 expression.关键词
大豆苷元/人乳牙牙髓干细胞/增殖/成骨分化Key words
Daidzein/ Stem cells derived from human exfoliated deciduous teeth/ Proliferation/ Osteogenicdifferentiation分类
医药卫生引用本文复制引用
武斌,孟秋菊,谢春,黄谦,魏波,陈敏莹..大豆苷元对人乳牙牙髓干细胞增殖和成骨分化的影响[J].昆明医科大学学报,2012,33(9):11-14,25,5.
