昆明医科大学学报2012，Vol.33Issue(9)：59-62,4.
高效液相色谱法测定万古霉素的血药浓度
HPLC Determination of Vancomycin in Human Plasma
郑巧玲 1张峻 1姚勤 1黄桦1
作者信息
- 1. 昆明医科大学第一附属医院临床药学中心,云南昆明650032
- 折叠
摘要
Abstract
Obejective To establish a high performance liquid chromatography (HPLC) method for the determination of concentration of vancomycin in human plasma. Methods Norvancomycin was used as the internal standard. The chromatography was performed on a Hypersil BDS C8 (4.6 mm X 250 mm, 5(un) column with the mobile phase consisted of 20 mmol/L potassium dihydrogen phosphate-acetonitrile, gradient elution was used, the flow rate was 1.0 mL/min, UV detection was set at 236 nm and column temperature was 40 t. RSSLlltS The calibration curve was linear within the range of 1.313 ~ 105 mg/L (r= 0.991). The intra- and inter-day precisions were less than 5.2%, the absolute extraction recoveries were more than 66%. Conclusion HPLC is a rapid, simple and accurate method for detecting the plasma concentration of vancomycin.关键词
万古霉素/高效液相色谱法/血药浓度Key words
Pancomycin/ HPLC/ Plasma drug concentration分类
医药卫生引用本文复制引用
郑巧玲,张峻,姚勤,黄桦..高效液相色谱法测定万古霉素的血药浓度[J].昆明医科大学学报,2012,33(9):59-62,4.
