口腔颌面外科杂志2012，Vol.22Issue(5)：312-317,6.DOI:10.3969/j.issn.1005-4979.2012.05.002
GLUT1基因有效siRNA序列的筛选实验研究
Experiment Study on Screening of Effectual siRNA-mediated GLUT1
摘要
Abstract
Objective: This study aimed to design and synthesize small interfering RNA ( siRNA) targeting glucose transporter-1 (GLUT1), and further screen the siRNA which can specifically and effectively suppresses GLUT1 expression. Methods: Four GLUT1 specific double stranded siRNAs were designed and electroporated into Cal-27 cells by Lipofectamine?2000 in 293T cells. The transcription level of GLUT1 were analyzed by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR), GLUT1 protein was tested by western blotting and glucose uptake inhibition rates were tested by 18F-FDG after GLUTl-siRNA. Results: Two of the four customized siRNA manifested effective inhibition on the expression of GLUT1. Conclusion: siRNA targeting GLUT1 can be successfully designed and synthesized, which can specifically and effectively suppresses the expression of GLUT1 gene and glucose uptake. The results may be beneficial to further study on gene therapy of tumors by RNA interfere (RNAi).关键词
葡萄糖转运蛋白1 (GLUT1)/RNA干扰/siRNA/肿瘤/基因治疗Key words
GLUT1/ RNAi/ siRNA/ tumor/ gene therapy分类
生物科学引用本文复制引用
王耀东,李生娇,廖建兴..GLUT1基因有效siRNA序列的筛选实验研究[J].口腔颌面外科杂志,2012,22(5):312-317,6.基金项目
国家自然科学基金资助项目(81102047) （81102047）
上海市自然科学基金资助项目(10ZR1432900) （10ZR1432900）
中央高校基本科研业务费专项资金资助项目 （）
