口腔颌面外科杂志2012，Vol.22Issue(5)：328-332,5.DOI:10.3969/j.issn.1005-4979.2012.05.005
干细胞因子在大鼠下颌骨缺损修复中的表达研究
Expression of Stem Cell Factor during the Process of Regeneration in Bone Defect of Mandibule
摘要
Abstract
Objective: The current paper was aimed to detect the expression of stem cell factor (SCF) during the repair process of rats' mandibular defects. Methods: 36 SD rats were used in this study. In one side of the mandible , critical size bone defect (CSD) in 5 mm dimension was established. A self-healing bone defect in 2 mm dimension was created in the contralateral side of the mandible. Animals were randomly divided into 6 groups and sacrificed on 1, 3, 5, 7, 14, 21 d after surgery respectively. Expression of SCF during the regeneration process were determined by immunohistochemistry and Western blot. Data were expressed as x眘 by SPSS 13.0 software. We used Student's t test to determine the significance of difference between groups. Significance was set at P<0.05. Results: The expression of SCF genes was detected in both groups at all times, and expression of SCF in CSD group was weaker than those in self-healing bone defect group at the same time (P<0.05). The results of western blot also confirmed the results by immunohistochemistry. Conclusion: SCF plays an important role in bone defect repair.关键词
干细胞生长因子/临界性骨缺损/自愈性骨缺损/鼠Key words
stem cell factor/ critical size defect/ self-healing size defect/ rat分类
医药卫生引用本文复制引用
徐丽,朱思姮,邹多宏,窦晓晨,周健..干细胞因子在大鼠下颌骨缺损修复中的表达研究[J].口腔颌面外科杂志,2012,22(5):328-332,5.基金项目
国家自然科学基金资助项目(81100788,81070864) （81100788,81070864）
安徽医科大学博士科研启动基金项目(XJ201109) （XJ201109）
安徽医科大学科研基金项目(2011xkj017) （2011xkj017）
