临床与实验病理学杂志2012，Vol.28Issue(10)：1091-1093,1097,4.
FISH技术在鉴别上尿路尿道上皮癌与良性疾病引起血尿中的作用
Roles of fluorescence in situ hybridization in differential diagnosis of urothelial carcinoma in upper urinary tract from benign diseases with hematuria
摘要
Abstract
Purpose To evaluate the clinical utility of a fluorescence in situ hybridization ( FISH ) assay as a non-invasively molecular test to distinguish urothelial carcinoma in upper urinary tract ( UUT-UC ) from benign lesions presenting with hematuria. Methods The chromosomal abnormalities of chromosomes 3,7, 17, and 9 ( pi 6 ) in hematuria specimens from 34 patients with UUT-UC and 33 patients with benign disorders were detected by using a set of fluorescently labeled DNA probes. The abnormalities of chromosomes were determined and analyzed between UUT-UC and benign disorders. Results The abnormalities of chromosomes were detected in 25 out of 34 ( 73. 5% ) patients with UUT UC and in 2 out of 33 ( 6. 1 % ) patients with benign disorders ( P ＜ 0. 001 ). Conclusions FISH assay of chromosomes 3, 7, 9, and 17 performed on exfoliated cells from voided urine specimens could be used as a non-invasive method to distinguish urothelial carcinoma in upper urinary tract from benign disorders presenting with hematuria.关键词
尿道肿瘤/尿道上皮癌/血尿/荧光原位杂交Key words
urethra! neoplasms/ urotheliai carcinoma/ hematuria/ fluorescence in situ hybridization分类
医药卫生引用本文复制引用
王建东,吴晋蓉,程文,王璇,时姗姗,周晓军..FISH技术在鉴别上尿路尿道上皮癌与良性疾病引起血尿中的作用[J].临床与实验病理学杂志,2012,28(10):1091-1093,1097,4.基金项目
国家自然科学基金(30970813、81171391) （30970813、81171391）
