环境与职业医学2012，Vol.29Issue(10)：629-631,3.
低强度微波对盐酸阿霉素致人早幼粒白血病细胞损伤的影响
Low Intensity Microwave Pre-exposure Alleviates Damages to HL-60 Cells Induced by Doxorubicin Hydrochloride
摘要
Abstract
[ Objective ] To explore the protective effects of low intensity microwave on doxorubicin hydrochloride (DOX) induced damages in HL-60 cells. [ Methods ] HL-60 cells were randomly divided into control, microwave alone, DOX alone, and combined exposure groups. The microwave alone and the combined exposure groups were exposed to 12μW/cm2 microwaves, 1 hour per day for 3 days. The cells in the control and the DOX alone groups were placed in the same condition, hut without microwave radiation. The combined exposure and the DOX alone groups were subsequently exposed to DOX at the concentration of 0.125 mg/L on the 4th day. Apoptosis was detected with Annexin V-FITC/PI double staining. Mitochondrial membrane potential (MMP) and intracellular Ca2+ concentrations were determined by flow cytometry. [ Results ] Compared with the control group, the apoptosis rate and Ca2+ concentration of the DOX alone group significantly increased (P < 0.05), while the MMP statistically decreased (P < 0.05). Compared with the DOX alone group, the apoptosis rate and Ca2* concentration of the combined exposure group significantly decreased (P<0.05), while the MMP statistically increased (P<0.05). [ Conclusion ] Under this experimental condition, pre-exposure with 12μW/cm2 900 MHz microwaves could significantly alleviate DOX-induced damages to HL-60 cells.关键词
微波/盐酸阿霉素/凋亡/线粒体膜电位/钙离子Key words
microwave/ doxorubicin hydrochloride/ apoptosis/ mitochondrial membrane potential/ Ca2+分类
医药卫生引用本文复制引用
刘蕴莹,靳宗达,姜秉成,周振,童建,曹毅..低强度微波对盐酸阿霉素致人早幼粒白血病细胞损伤的影响[J].环境与职业医学,2012,29(10):629-631,3.基金项目
江苏省放射医学与防护重点实验室开放课题(编号:KJS0930) （编号:KJS0930）
