棉花学报2012，Vol.24Issue(5)：414-419,6.
陆地棉GhSPL3基因的克隆、亚细胞定位及表达分析
Cloning,Subcellular Localization and Expression Analysis of GhSPL3 Gene in Gossypium hirsutum L
摘要
Abstract
GhSPL3, an SPL transcription factor, was cloned from upland cotton by bioinformation and RT-PCR technology. The sequence accession number is JN795132 in GenBank. The ORF length of GhSPLi is 426 bp which encodes 141 amino acid residues. Bioinformatics analysis showed that GhSPLi contains a typical SBP structure domain and a nuclear localization signal; Evolutionary tree analysis revealed that GhSPLi and AtSPLi were clustered in same group, thus GhSPLi and AtSPLi might have similar structure and function. Subcellular localization results showed that the signal of GhSPLi was localized in the nucleus. Quantitative RT-PCR results showed that GhSPLi expressed in all tissues, but the expression levels were different. The expression of GhSPLi in the flowers was the highest, and the lower was that in shoot apexes and in stem, followed by that in the root and in the leaves. Among different development stages of shoot apex, the expression of GhSPLi was the highest at the third true leaf expansion stage in shoot apex. The result of Quantitative RT-PCR indicated that GhSPLi might play an important role in bud differentiation, the transition of the growth phase and flower formation.关键词
陆地棉/GhSPL3/亚细胞定位/荧光定量RT-PCRKey words
Gossypium hirsutum L./GhSPLi/ subcellular localization/ quantitative RT-PCR分类
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李洁,范术丽,宋美珍,庞朝友,喻树迅..陆地棉GhSPL3基因的克隆、亚细胞定位及表达分析[J].棉花学报,2012,24(5):414-419,6.基金项目
国家高技术研究发展计划(2011AA10A102) （2011AA10A102）
国家棉花产业技术体系(CARS-18) （CARS-18）
