国际眼科杂志2012，Vol.12Issue(9)：1614-1618,5.DOI:10.3969/j.issn.1672-5123.2012.09.02
整合素连接激酶对人视网膜色素上皮细胞增殖和迁移的影响
Effect of integrin-linked kinase on the proliferation and migration of human retinal pigment epithelium cells
张奕霞 1张晓阳 2陈宁宁 1李彩云1
作者信息
- 1. 1830008,中国新疆维吾尔自治区石河子市,新疆石河子大学医学院第一附属医院眼科
- 2. 830000,中国新疆维吾尔自治区乌鲁木齐市,新疆医科大学第五附属医院老年病内科
- 折叠
摘要
Abstract
AIM: To investigate the effect of RNA interference targeting integrin-linked kinase (ILK) on the proliferation and migration of cultured human retinal pigment epithelium (hRPE)cells. were determined. ILK specific siRNA was designed and synthesized. siRNA was transfected into hRPE cells with Lipofectamine 2000. The effects of transfection were observed using a fluorescence microscope. RT-PCR and Western blot were used to semiquantitatively evaluate the mRNA and protein expressions of ILK. MTT assay was used to detect the proliferation of hRPE cells. Wound healing assay and transwell migration assay were used to detect the migration capability of hRPE cells.expressions of ILK significantly decreased by siRNA transfection(P<0.01).The proliferation of hRPE cells also significantly decreased(P<0.05). The migration capability of hRPE significantly decreased by siRNA transfection,with statistically significant difference compared with the normal control group(P<0.05). expressions of ILK,as well as the proliferation and migration abilities of hRPE cell's.关键词
RNA干扰/整合素连接激酶/视网膜色素上皮细胞/增殖/迁移Key words
RNA interference/ILK/retinal pigment epithelium/migration/proliferation引用本文复制引用
张奕霞,张晓阳,陈宁宁,李彩云..整合素连接激酶对人视网膜色素上皮细胞增殖和迁移的影响 [J].国际眼科杂志,2012,12(9):1614-1618,5.
