生态与农村环境学报2012，Vol.28Issue(6)：700-705,6.
有机污染场地浅层地下水对斑马鱼胚胎发育的生物毒性研究
Bio-Toxicity of Shallow Groundwater in Organic Pollutants Contaminated Site to the Development of Embryos of Zebrafish
摘要
Abstract
Zebrafish was chosen as a subject in tests on shallow groundwaters ( GW1 , GW2 and GW3) sampled from 3 different sites, a hazardous chemical storage, chlorinated paraffin workshop and a place outside the chemical plant, for a-cute toxicity to zebrafish and toxicity affecting the development of zebrafish embryos. Results show that to adult zebrafish φ(72 h,LC50) of GW1 was 79. 12% , and of GW2 and GW3 both greater than 100%. Different degrees of toxic effects of the three shallow groundwaters on development of zebrafish embryos were recorded. The biotoxicity indices, like egg coagulation rate in 24 h, non-spontaneous movement within 20 s and hatching rate in 72 h, were found to be closely related to dosage of the pollutant. The waters also had certain teratogenic effects on zebrafish, which would display pericardial cyst, spinal curvature, tail curvature or lack of melanin. The waters followed an order of GW 1 > GW 2 > GW 3 in biotoxicity. It is suggested that the toxicity test on zebrafish embryos is higher in sensitivity than the acute toxicity test. Moreover, the former is simple and rapid, and hence can be used in rapid screening of contaminated groundwater, which provides scientific basis for further hazard identification and risk assessment.关键词
污染场地/浅层地下水/斑马鱼胚胎/生物毒性/污染诊断Key words
contaminated site/shallow groundwater/zebrafish embryo/bio-toxicity/pollution diagnosis分类
资源环境引用本文复制引用
周艳,徐建,冯艳红,赵欣,万金忠,林玉锁..有机污染场地浅层地下水对斑马鱼胚胎发育的生物毒性研究[J].生态与农村环境学报,2012,28(6):700-705,6.基金项目
国家高技术研究发展计划(2009AA063103) （2009AA063103）
环保公益性行业科研专项(200809100,200909075) （200809100,200909075）
