南京工业大学学报（自然科学版）2012，Vol.34Issue(6)：63-68,6.DOI:10.3969/j.issn.1671-7627.2012.06.013
吐温60与纤维素酶协同超声波法提取红藤中总黄酮的工艺
Extraction process of flavonoids from Sargentodoxa by ultrasonic extraction assisted with Tween-60 and cellulase
摘要
Abstract
Ultrasonic extraction assisted with Tween-60 and celluiase was adopted to extract the flavonoids from Sargentodoxa. Factors influencing the extraction yield and the antioxidability property, such as the kind and the concentration of surfactant, the kind and the concentration of enzyme, extractive temperature and duration,solvent pH value, and the liquid to solid ratio were investigated. The optimal conditions were verified by the orthogonal experiment. The conditions for the optimal flavonoids extraction yield of 7. 98% were extractive duration 70 min,liquid to solid ratio 30 mL/g,concentration of celluiase 0. 5 mg/mL. As for antioxidability,the optimal conditions were extractive duration 70 min,liquid to solid ratio 50 mL/g,and concentration of celluiase 0. 5 mg/mL,and the free radical scavenging capacity was 67. 56% .关键词
吐温60/纤维素酶/超声波/红藤/黄酮Key words
Tween-60/ celluiase/ ultrasonic/ Sargentodoxa/flavonoid分类
医药卫生引用本文复制引用
韩伟,叶亚婧,葛珊珊,姬鹏俊,雷祖海..吐温60与纤维素酶协同超声波法提取红藤中总黄酮的工艺[J].南京工业大学学报（自然科学版）,2012,34(6):63-68,6.基金项目
国家大学生创新性实验计划资助项目(101025112) （101025112）
