南京林业大学学报（自然科学版）2012，Vol.36Issue(5)：19-25,7.
利用ISSR和SRAP标记分析油茶遗传多样性
Genetic diversity of Camellia oleifera using ISSR and SRAP markers
摘要
Abstract
In this paper, two kinds of molecular markers, ISSR and SRAP were used to assess the genetic diversity in 192 accessions of Camellia oleifera. Ten primers were screened from 100 primers of ISSR, 118 bands were amplified, a-mong which 104 bands were polymorphic bands, the percentage of polymorphic band was 88. 14 % , the Nei's genetic similarity coefficients ranged from 0. 52 to 1. 00. Twelve pairs primers of SRAP were selected out, 284 bands were amplified , of which 244 bands (85. 37 % ) were polymorphic, and the Nei's genetic similarity coefficients ranged from 0. 65 to 0. 95. The correlation of the genetic similarity matrix among the ISSR, SRAP and the combination data were higher. It indicated that the two methods had highly consistent in the genetic diversity analysis of C. oleifera, furthermore the SRAP technique was more reliable. According to the UPGMA method, we made the cluster analysis by using the NTSYS software. The 192 germplasms of C. oleifera could be divided into 6 groups at the similarity coefficient of 0. 68 by ISSR marker; they also could be divided into 6 groups at the similarity coefficient of 0. 70 by SRAP marker. This indicated that the C. oleifera germplasms had close relationship. However, the dendrogram's cluster analysis showed that some germplasms probably represent different genetic background and high genetic diversity.关键词
油茶/遗传多样性/ISSR/SRAPKey words
Camellia oleifera/ genetic diversity/ ISSR/ SRAP分类
农业科技引用本文复制引用
彭方仁,吴莺莺,郝明灼,陈隆升,陈永忠..利用ISSR和SRAP标记分析油茶遗传多样性[J].南京林业大学学报（自然科学版）,2012,36(5):19-25,7.基金项目
"十一五"国家科技支撑计划(2009BADB1B04) （2009BADB1B04）
