南京林业大学学报（自然科学版）2012，Vol.36Issue(5)：31-36,6.
利用杨树原生质体瞬时表达系统筛选杨生褐盘二孢菌效应因子蛋白
Identification of Marssonina brunnea f.sp. multigermtubi effector proteins using poplar protoplast transient expression system
摘要
Abstract
Pathogenic fungi effector proteins play an important role in infecting their host plants. At present, identification and functional studies of the effector proteins are the emphasis and difficulty for disease resistance research in plants. Based on Marssonina brunnea f. sp. multigermtubi genome sequence and structural characteristics of fungal effector proteins, we predicted 106 candidate effectors, and 25 of them were successfully cloned. Then, we used poplar protoplast transient expression system to study the intracellular function of these 25 candidate effectors, and 4 of them were identified to inhibit the activity of poplar transcription factor WRKY29 promoter, which is involved in poplar immune system. The results indicated that these 4 effector proteins might play important roles in disturbing the signal transduction pathway of plant immune system.关键词
杨树原生质体瞬时表达系统/WRKY29/杨生褐盘二孢菌/效应因子Key words
poplar protoplast transient expression system/ WRKY29/ Marssonina brunnea f. sp. multigermtubi/ effector分类
农业科技引用本文复制引用
曹友志,谭碧玥,王明庥,黄敏仁..利用杨树原生质体瞬时表达系统筛选杨生褐盘二孢菌效应因子蛋白[J].南京林业大学学报（自然科学版）,2012,36(5):31-36,6.基金项目
国家重点基础研究发展计划(2009CB119100) （2009CB119100）
