南京林业大学学报（自然科学版）2012，Vol.36Issue(5)：47-50,4.
青钱柳幼胚愈伤组织的诱导
Callus induction from young embryos of Cyclocarya paliurus
摘要
Abstract
Using young embryos of Cyclocarya paliurus as explants, we investigated the effects of embryo development period, basic media, types and the concentrations of plant growth regulators on callus induction of C. paliurus. The results showed that there were obvious differences in the shape of callus among different embryo development periods, and the middle cotyledon embryos were most suitable for callus induction. As a basic media, improved DKW medium was the best selection for young embryo callus induction, of which induction rate could hit to 88. 89 % with no browning phenomenon. The medium containing 2, 4 - D and KT was more appropriate for callus induction. The 2, 4 - D (0. 5 mg/L) + KT (2.0 mg/L) as well as 2,4 - D (1.0 mg/L) + KT (1. 0 mg/L) were optimal for callus induction of C. paliurus young embryo, and the induction rates were 80.00 % and 78.33 % respectively.关键词
青钱柳/幼胚/愈伤组织/培养基/植物生长调节剂Key words
Cyclocarya paliurus/ young embryo/ callus/ media/ plant growth regulator分类
农业科技引用本文复制引用
张金凤,方升佐,尚旭岚,徐锡增..青钱柳幼胚愈伤组织的诱导[J].南京林业大学学报（自然科学版）,2012,36(5):47-50,4.基金项目
江苏高校优势科技创新团队科研计划(苏教科[2009]10号) （苏教科[2009]10号）
国家林业局林业科学技术推广项目([2011]18号) （[2011]18号）
国家林业公益性行业科研专项项目(200904046) （200904046）
