内科理论与实践2012，Vol.7Issue(5)：378-383,6.DOI:10.3969/j.issn.1673-6087.2012.05.014
整合素β3胞浆段尾部序列对αvβ3介导的细胞行为的影响
Differential roles of amino acid sequences of integrin β3 cytoplasmic tail in αvβ3-mediated cell behaviors
摘要
Abstract
Objective To study the effect of integrin β3 subunit cytoplasmic domain on adhesion, spreading and migration of tumor cells, and providing insights into the molecular targets for interfering the progress of tumor. Methods Stable Chinese hamster ovary (CHO) cell lines that co-express human integrin αv and full length β3 or mutant β3 with truncation at COOH terminal T758 or Y759were established. Spreading and adhesion of CHO-αvβ3, CH0-αvβ3 △758 and CHO-αvβ3 △ 759 cells on immobilized vitronectin were examined and transwell assay was performed to evaluate the migration ability of the cell lines. Results The CHO cells transfected with integrin av and wild-type integrin β3 (CHO-αvβ3 cells) had the ability of attachment, spreading and migration on immobilized vitronectin. Compared with CIIO-αvβ3 cells, CHO cells transfected with integrin av and inlegrin β3 truncated at T758 (CHO-αvβ3 △758 cells) failed to attach, spread or migrate on immobilized vilronectin, while CHO cells tranfected with integrin av and inlegrin β3 truncated at Y759 (CH0-αvβ3 △759 cells) were able to attach on immobilized vitronectin but with an impaired ability of spreading and migration. Conclusions The integrin β3 subunit cytoplasmic domain plays an important role in adhesion, spreading and migration of αvβ3-expressing cells, and in which the RGT and YKGT sequences of integrin β3 cytoplasmic tail act differentially. Data suggest that these sequences may represent the therapeutic targets for treatment of tumor.关键词
整合素αvβ3/截短体/黏附/迁移Key words
Integrin αvβ3/ Truncation/ Adhesion/ Migration分类
医药卫生引用本文复制引用
周玉兰,黄建松,刘萍,施小凤,崔雄鹰,阮铮,奚晓东..整合素β3胞浆段尾部序列对αvβ3介导的细胞行为的影响[J].内科理论与实践,2012,7(5):378-383,6.基金项目
国家自然科学基金(项目编号:81070414) （项目编号:81070414）
