内科理论与实践2012，Vol.7Issue(6)：434-438,5.DOI:10.3969/j.issn.1673-6087.2012.06.010
铜绿假单胞菌在急性下呼吸道感染患者中生物被膜形成与群体感应系统基因相关性分析
Analysis the correlation between biofilm formation of Pseudomonas aeruginosa and quorum-sensing system gene in patients with acute lower respiratory tract infection
摘要
Abstract
Objective To investigate the correlation between expression of Las and Rhl signal system in quorum-sensing (QS) system and biofilm formation in Pseudomonas aeruginosa acute lower respiratory tract infection. Methods To detect the relative gene expression of Las and Rhl signal system and biofilm forming ability, analyzing the correlation between expression of Las and Rhl system and biofilm forming ability. Results Of the 120 clinically isolated Pseudomonas aeruginosa strains, expression of Las signal system LasI and LasR relative gene was positively correlated with biofilm formation ( P<0.001 ); the first day clinical isolated strains had their LasI and LasR relative gene expression positively correlated with biofilm formation (P<0.01), day 14 clinical isolated strains had their LasI relative gene expression closely positive correlated with biofilm formation (P<0.001) and LasR relative gene expression positively correlated with biofilm formation (P<0.05). Rhl signal system RhlI and RhlR relative gene expression was closely positive correlated with biofilm formation (P<0.001); the first day RhlI and RhlR relative gene expression and biofilm formation was closely positive correlated (P<0.001), and the 14 days Rhll and RhlR relative gene expression was not correlated with biofilm formation. Conclusions Las and Rhl signal system in QS system had a close correlation with biofilm formation and played a key role in the process of biofilm formation in Pseudomonas aeruginosa.关键词
铜绿假单胞菌/群体感应系统/生物被膜Key words
Pseudomonas aeruginosa/ Quorum-sensing/ Biofilm分类
医药卫生引用本文复制引用
李镛,刘嘉琳,瞿洪平..铜绿假单胞菌在急性下呼吸道感染患者中生物被膜形成与群体感应系统基因相关性分析[J].内科理论与实践,2012,7(6):434-438,5.基金项目
上海交通大学医学院附属瑞金医院优秀青年教师资助项目 （）
