农业生物技术学报2012，Vol.20Issue(10)：1159-1167,9.DOI:10.3969/j.issn.1674-7968.2012.10.008
优化的小鼠STO细胞系重编程多功能干细胞的建立
Establishing of the Optimization of Mouse (Mus musculus) STO Cell Lines Reprogram to Pluripotent Stem Cells
摘要
Abstract
Research of the induced pluripotent stem cells (iPSCs) by inducing the SIM-6-thiogunanie-oualiain (STO) cells. Lentivirus vector FUW-OSKM which included octamer-binding transcription factor 4(Oct4), SRY (sex determining region Y)-box 2(Sox2), Kruppel-like factor 4 (Klf4) and c-Myc transfected to 293FT cells by calcium phosphate method. Then, using the virus infected STO cells after lentivirus was packaged and the cells were cultured in the ESC media. The clones were isolated on the fifteenth day cultured on feed layer and none feed layer culture dishs.Through the biological characteristics analysis, we found the cells named STO-iPSCs had the typical morphological characteristics of embryonic stem cells, had a positive result by alkaline phosphatase staining,expressed embryonic stem cells marker Nanog and Oct4 by the immunocytochemistry and high quantitys of Nanog and Oct4 mRNAs by qPCR, especially could differentiate to nerve cells in vitro. In conclution, we obtained STO-iPSCs and stablished the STO-iPSCs training system without raising layer.关键词
慢病毒载体/STO细胞/诱导多功能干细胞(iPSCs)/胚胎干细胞Key words
Lentivirus vector,SIM-6-thiogunanie-oualiain (STO) cells,induced pluripotent stem cells(iPSCs),Embryonic stem cells引用本文复制引用
权富生,刘琴,吴月红,吴海波,张涌..优化的小鼠STO细胞系重编程多功能干细胞的建立[J].农业生物技术学报,2012,20(10):1159-1167,9.基金项目
国家高技术研究发展计划(863)项目(No.2011AA100303) （863）
