热带作物学报2012，Vol.33Issue(9)：1608-1613,6.DOI:10.3969/j.issn.1000-2561.2012.09.015
龙眼胚性愈伤组织fw2.2家族2个基因的克隆与分析
Cloning and Sequence Analysis of Two Members of fw2.2 Gene Family from Embryogenic Callus in Dimocarpus longan Lour
摘要
Abstract
fruit weight 2.2 (fw>2.2) is an important quantitative trait locus (QTL) gene in controlling the fruit weight of plants. Based on the database of longan transcriptome, two members of fu>2.2 family genes from longan embryogenic callus were obtained by the homologous cloning and RACE technology. They were named as Dlfw2.2-1 and Dlfw2.2-2, respectively. Bioinformatics ananlyses showed that the complete cDNA sequences of Dlfw2.2-1 were 970 bp, encoding 184 amino acids and the Dlfiv2.2-2 were 941 bp, encoding 175 amino acids. Both the nucleotides and amino acids sequences of the two members were high homologous with those of the known fw2.2 genes in other species. Their proteins had no signal peptide, locating in the plasma membrane with transmembrane structure and the typical cysteine-rich protein domain. Phylogenetic tree analyses indicated that Dlfw2.2-1 and Dlfw2.2-2 belonged to the same branch with a close genetic relationship with Lycopenicon esculentum and Persea amerscarea.Therefore, the 2 members putatively belonged to the fw.2 family.关键词
龙眼/胚性愈伤组织/fw2.2/果实重量/基因克隆/生物信息学分析Key words
Dimocarpus longan/ Embryogenic callus/ fw2.2/ Fruit weight/ Gene cloning/ Bioinformatics analyzing分类
农业科技引用本文复制引用
陈裕坤,林玉玲,赖钟雄..龙眼胚性愈伤组织fw2.2家族2个基因的克隆与分析[J].热带作物学报,2012,33(9):1608-1613,6.基金项目
国家自然科学基金项目(No.31078717) （No.31078717）
高等学校博士学科点专项科研基金(No.20093515110005) （No.20093515110005）
福建省科技平台建设项目(No.2008N2001). （No.2008N2001）
