热带作物学报2012,Vol.33Issue(9):1663-1669,7.DOI:10.3969/j.issn.1000-2561.2012.09.025
六棱景天组织培养与快速繁殖研究
Tissue Culture and Rapid Propagation of Sedum sexangulare
摘要
Abstract
In this experiment the potted plants were used as the materials to establish the aseptic system and to study the effects of different hormone combinations, basic mediums, light intensities, light qualities and concentrations of agars on the proliferation and rooting in Sedum sexangulare, to compare the different effects of transplanting mediums on the transplanting survival rates. The obtained results showed that the MS medium with NAA 0.1 mg/Land6-BA l.Omg/Lwas good for the induction of axillary buds and formation of bud clusters. The best hormone combination for proliferation was NAA 0.1 mg/L and 6 -BA 2.0 mg/L, and the albefaction or vitrification phenomenon was not prone to occur when the basal medium MS with 0.8% agar was used. The proliferation and growth were greatly influenced by the light quality, which were improved in the three colors of lights (red, blue, white)and weakened in green light. The best rooting medium was 1/4 MS with NAA 0.05 mg/L and IBA 0.01 mg/L when the light intensity was 1 000 Ix. And the highest survival rate of transplanting was 84.39% in complex substrate(vermiculite : peat=l : 1).关键词
六棱景天/增殖/生根/移栽/快速繁殖Key words
Sedum sexangulare/ Proliferation/ Rooting/ Transplanting/ Rapid propagation分类
农业科技引用本文复制引用
叶秀妹,肖世明,杨露云,吴露莉,蔡永红,冯新,赖钟雄..六棱景天组织培养与快速繁殖研究[J].热带作物学报,2012,33(9):1663-1669,7.基金项目
福建省亚热带果树及特种经济作物种质资源共享平台(No.2008N2001) (No.2008N2001)
国家科技支持计划(No.2007BAD07B03)资助项目的部分内容. (No.2007BAD07B03)