热带作物学报2012，Vol.33Issue(9)：1686-1689,4.DOI:10.3969/j.issn.1000-2561.2012.09.029
贵州省白术白绢病菌遗传多样性评价
Genetic Diversity Analysis of Sclerotium rolfsii Sacc.in Rhizoma of Atractylodes Macrocephala
摘要
Abstract
The genetic diversity of Sclerotium rolfsii Sacc. in Atractylodes macrocephala was studied in order to analyse the relationship between the strains from 5 regions (Guiyang, Zunyi, Qiandongnan, Qiannan and Bijie city)in Guizhou Province. 20 strains collected from different biotopes were amplified by 116 RAPD primers. The genetic relationships were generated using UPGMA method of NTSYS software based on the DNA fingerprint. 64 bands including 44 reproducible polymorphic bands with 68.75% polymorphic band frequency based on 12 RAPD primers were obtained . The similarity coefficient between strains was from 0.56 to 0.98, which were divided into 3 groups marked I, II and M based the threshold of similarity coefficient at 0.79. The results indicated that DNA polymorphism had no significant correlation with geographical origins, excepting a few of strains from Bijie were divided into a same group.关键词
罗氏小核菌/RAPD/NTSYS-PC分析/遗传多样性Key words
Sclerotium rolfsii Sacc/ RAPD/ NTSYS-PC clustering analysis/ Genetic diversity分类
农业科技引用本文复制引用
肖仲久,李小霞,蒋选利..贵州省白术白绢病菌遗传多样性评价[J].热带作物学报,2012,33(9):1686-1689,4.基金项目
贵州省科技厅自然科学基金项目(黔科合J字[2009]2104号) （黔科合J字[2009]2104号）
贵州省遵义市科技支撑计划(社会发展攻关)项目(遵市科合社字2009-24号) （社会发展攻关）
遵义师范学院博士启动基金项目(No.2012BSJJ09). （No.2012BSJJ09）
