四川大学学报（自然科学版）2012，Vol.49Issue(5)：1107-1114,8.DOI:10.3969/j.issn.0490-6756.2012.05.029
利用酵母双杂交系统筛选与BnSmD1相互作用的蛋白
Screening of the proteins interacting with the BnSmD1 by Yeast Two-Hybrid System
摘要
Abstract
From the Brassica napus cDNA library, BnArf GAP1 (Brassica napus ADP-ribosylation factor GTPase activating proteinl), wihch interacted with the bait protein, BnSmDl, was obtained by yeast two-hybrid system screening. By reverse transcription PCR, the open reading frames of three Arf GAP genes in Brassica napus were cloned, which were named as BnArf GAP1, BnArf GAP2 and BnArf GAP3, respectively. Both BnArf GAP1 and BnArf GAP 2 possessed an open reading flame of 507 bp, encoding a protein of 168 amino acid residues. BnArf GAP3 possessed an open reading frame of 249 bp, encoding a protein of 82 amino acid residues, because its nucleotide sequence was inserted by an unhomologous 93bp sequence with a stop codon, which caused the early termination of translation. The nucleotide sequences of BnArf GAP 1 and BnArf GAP2 had 98. 22% similarity. The nucleotide sequences of BnArf GAPS and the other two genes had 82. 83% and 84.17% similarity, respectively. All of the three proteins contained a C2 domain.关键词
酵母双杂交/BnSmD1/BnArf GAP/C2结构域Key words
yeast two-hybrid system/ BnSmD1/ BnArf GAP/ C2 domain分类
生物科学引用本文复制引用
张克,汪静,邓薇,刘洋,赵云..利用酵母双杂交系统筛选与BnSmD1相互作用的蛋白[J].四川大学学报（自然科学版）,2012,49(5):1107-1114,8.基金项目
国家自然科学基金(30871540) （30871540）
