四川大学学报（自然科学版）2012，Vol.49Issue(5)：1137-1142,6.DOI:10.3969/j.issn.0490-6756.2012.05.033
珙桐花药诱导愈伤组织的初步研究
Preliminary study on the Davidia involucrata anther callus induction
摘要
Abstract
The anthers of the endangered plant Davidia involucrata were chosed as explants for the first time to induce anther callus in this study. The relationship between bud appearances and developmental stages of microspores was identified first. And the anthers were cultured in MS mediums contain different hormone types, concentrations and sugar concentrations. The result indicated that the effect of low-temperature pretreatment on callus is not significant. The callus inductivity is decreased gradually with the extending of low-temperature treatment time. It also indicated that high concentration of sugar is bad for the formation of callus. The best medium to induce anther callus in this study is using 1. 7~2. 2 cm long bud, 4 ℃ pretreated 2 day and the medium MS4-2,4-D 1 mg/L+NAA 0. 5 mg/L+GA3 lmg/L + 5% sugar; the highest inductivity is 91. 3. After the callus induction, some explorations of the induction of bud had been done. However, due to the serious browning of callus, the differentiation was unsuccessful.关键词
珙桐/花药培养/愈伤组织Key words
davidia/anther culture/callus分类
农业科技引用本文复制引用
陈蕤坤,吴鹏飞,何青霞,周海滨,黄治宇,徐莺..珙桐花药诱导愈伤组织的初步研究[J].四川大学学报（自然科学版）,2012,49(5):1137-1142,6.基金项目
国家科技基础性工作专题重点项目(2008FY110400) （2008FY110400）
大学生创新训练计划重点项目(20120226) （20120226）
