四川大学学报（自然科学版）2012，Vol.49Issue(5)：1143-1148,6.DOI:10.3969/j.issn.0490-6756.2012.05.034
调节细胞自噬对鱼藤酮诱导PC12细胞损伤的影响
Effects of adjusting autophagy on rotenone induced PC12 cells damage
摘要
Abstract
To detect the effects of adjusting autophagy on rotenone induced PC12 cells apoptosis, PC12 cells treated by rotenone were used as an in vitro model of Parkinson's disease, and, using rapamycin as the autophagy enhancer, Bafilomycin Al and 3-MA as inhibitor of autophagy. It was found that Bafilo-mycin Al greatly increased the apoptosis of cells,and 3-MA reduced the apoptosis of rotenone-induced cells, and rapamycin may contribute to impaired apoptosis, but reduce the damaged cells in early mortality. Construction PC12 cells of stably transfected pEGFP-LC3, flow cytometry results show, inhibit or promote autophagy, cell autophagy related protein LC3 were significantly increased.关键词
帕金森病/自噬/鱼藤酮/PC12细胞Key words
Parkinson's disease/ autophagy/ Rotenone/ PC12 cells分类
生物科学引用本文复制引用
张红,刘湧,金家贵,杨淑霞,张芹,刘进,王建东..调节细胞自噬对鱼藤酮诱导PC12细胞损伤的影响[J].四川大学学报（自然科学版）,2012,49(5):1143-1148,6.基金项目
成都医学院基金项目(CYZ11-007) （CYZ11-007）
四川省教育厅青年基金项目(10ZB096) （10ZB096）
