水产学报2012，Vol.36Issue(9)：1322-1330,9.DOI:10.3724/SP.J.1231.2012.27856
大黄鱼22个微卫星标记在F1家系中的分离方式及与生长性状的相关分析
The segregation patterns of 22 microsatellite markers in an F1 family of large yellow croaker( Larimichthys crocea)and correlation analysis with growth-related traits
摘要
Abstract
The large yellow croaker Larimichthys crocea is one of the most important fish species in Chinese mariculture, and the research about correlation analysis between growth-related traits and microsatellite markers is very rare. In this study, the genotype distribution of twenty-two microsatellite markers was investigated in an F1 family of large yellow croaker Larimichthys crocea of 20-month old, and the correlation between growth-related traits and microsatellite markers was also analyzed using statistical method. The results are as follows; A total of 60 alleles were detected, and the average number of alleles and effective number of alleles were 2. 73 and 2. 37 , respectively. The average observed heterozygosity (Ho) and expected heterozygosity (He) were 0. 75 and 0. 73, respectively. The genotype distribution of partly loci deviated severely from Mendel' s Laws, which suggested these loci might link with the adaptive gene, and LYC0446 may link with recessive homozygous lethal genes. We discovered that LYC0077 had significant impact on body weight,body length and body height(P ＜0. 05) ,and the allele A( 165 bp)had highest phenotype value and can be used as a molecular marker in selection breeding; LYC0015 and LYC0243 had significant impact on body height(P＜0. 05) ,and no significant effect on body length and body weight, and the allele C( 110 bp)for LYC0015 and the allele A(160bp)for LYC0243 have positive effect on growth-related traits. Three genotypes of these loci with favorable effect on growth-related traits were also determined, BC for LYC0015 , AA for LYC0017 and AB for Z,yC0243 respectively. The best genotype combinations ( BC/AA/AB ) for LYC0015 ,LYC0077 and LYC0243 were also selected according to body weight, which conformed to the Additive Effects Model. The results presented here provide a starting point for the eventual mapping of economically important genes and marker-assistant selection (MAS) in large yellow croaker.关键词
大黄鱼/微卫星标记/生长性状/相关分析Key words
Larimichthys crocea/ microsatellite markers/ growth-related traits/ correlation analysis分类
生物科学引用本文复制引用
刘贤德,韦信键,蔡明夷,刘洋,王志勇..大黄鱼22个微卫星标记在F1家系中的分离方式及与生长性状的相关分析[J].水产学报,2012,36(9):1322-1330,9.基金项目
国家"八六三"高技术研究发展计划(2012AA10A403) （2012AA10A403）
公益性行业(农业)科研专项(200903029-4) （农业）
国家自然科学基金项目(31172397) （31172397）
浙江海洋学院海洋渔业科学与技术浙江省重中之重学科开放课题(20100203) （20100203）
