水产学报2012，Vol.36Issue(9)：1450-1456,7.DOI:10.3724/SP.J.1231.2012.27862
哈维氏弧菌的分子遗传多样性研究
Molecular genetic diversity of Vibrio harveyi
摘要
Abstract
Vibrio harveyi is known to be a major pathogen in mariculture animal systems. Disease outbreaks attributed to V. harveyi have been reported in main mariculture animal farm production. In recent years,mass mortalities of many cultured kinds of maricultured fishes, such as Litopenaeus vannamei, Pseudosciaena crocea, and Lutjanus sanguineus have occurred frequently infected by V. harveyi. In this article the 101 isolates of V. harveyi were subjected to random amplified polymorphic DNA (RAPD)-PCR and BOX-PCR analysis to investigate the genetic variability among V. harveyi strains. A total of 10 RAPD primers were designed for their specificity in detecting V. harveyi, and only the primer PM2 was highly reproducible and found suitable to use in RAPD-PCR. The genetic diversity among V. harveyi isolates assessed by RAPD-PCR by PM2 primer yielded 15 different RAPD patterns which clustered the isolates into 18 groups at 65% similarity level. Similarly, BOX-PCR clustered the 20 patterns into 15 groups at 40% similarity. However, with RAPD-PCR and BOX-PCR, 101 V. harveyi could be divided into 18 groups at 50% similarity. We could obtain the accurate results of molecular genetic diversity by both RAPD-PCR and BOX-PCR.关键词
哈维氏弧菌/海水鱼类/BOX分型/RAPD分型Key words
Vibrio harveyi/ marine fish/ BOX typing/ RAPD typing分类
生物科学引用本文复制引用
徐芝亮,吴灶和,简纪常,黄郁葱..哈维氏弧菌的分子遗传多样性研究[J].水产学报,2012,36(9):1450-1456,7.基金项目
广东省科技计划国际合作项目(2009B050700040) （2009B050700040）
国家"九七三"前期专项(2011CB111601) （2011CB111601）
广东省科技计划重大项目(20080100014) （20080100014）
