水产学报2012，Vol.36Issue(9)：1457-1464,8.DOI:10.3724/SP.J.1231.2012.27944
一株病原发光杆菌的分离与鉴定
Isolation and identification of pathogenic Photobacterium sp.
摘要
Abstract
Dominant bacteria strain ( SX1 ) was isolated from diseased and dead megalopas Portunus trituberculatus, and have pathogenicity to healthy megalopa and juvenile ( Ⅲ) crab P. trituberculatus by artificial challenge. The phenotypic characteristics of the isolates including morphological, physiological and biochemical characteristics were determined. The 16S rRNA and two housekeeping genes,RNA polymerase a-chain (rpoA) and DNA gyrase B subunit ( gyrB) were sequenced, and the phylogenetic trees based on 16 S rRNA and gyrB sequences were constructed as an aid to identification. The sequenced 16 S rRNA gene of strain SX1 ( GenBank accession No. JQ394838) was 1 454 bp, the gyrB gene of strain SX1 ( GenBank accession No. JQ394839) was 1 077 bp.the rpoA gene(GenBank accession No. JQ394840) was 913 bp. The 16S rRNA,gyrB and rpoA genes were compared with sequences deposited in databases, and molecular phylogenetic analyses were conducted. The results showed that the characteristics of identified strain (SX1) consist with genus of Photobacterium,and the 16S rRNA,gyrB and rpoA genes of the identified strain(SXl) exhibited high similarity with Photobacterium sp. . The phylogenetic trees constructed using the neighbor joining method based on 16S rRNA and gyrB genes, strain SX1 clustered with the Photobacterium ganghwense, and was supported by a higher bootstrap value. Therefore, strain SX1 was similar to P. ganghwense. The results confirmed that the diseased megalopas were infected by P. ganghwense. To our knowledge, this is the first report of P. ganghwense as pathogenic bacteria of crab. The study showed the diversity and complexity of pathogenic bacteria from aquatic animals.关键词
三疣梭子蟹/Photobacterium ganghwense/16S rRNA基因/gyrB基因/rpoA基因Key words
Portunus trituberculatus/ Photobacterium ganghwense/ 16S rRNA/ gyrB/ rpoA分类
生物科学引用本文复制引用
徐加涛,阎斌伦,张晓君,白雪松,秦蕾,毕可然..一株病原发光杆菌的分离与鉴定[J].水产学报,2012,36(9):1457-1464,8.基金项目
江苏省水产三项工程项目(PJ2010-58,P2009-18) （PJ2010-58,P2009-18）
江苏高校优势学科建设工程项目 （）
连云港市科技攻关项目(CG1134) （CG1134）
