水产学报2012，Vol.36Issue(11)：1658-1666,9.DOI:10.3724/SP.J.1231.2012.27984
锦鲤酪氨酸酶基因序列分析及其在不同锦鲤品系不同组织中的表达
Analysis of tyrosinase gene and tissue expression in five different strains of Koi carp (Cyprinus carpio Koi)
摘要
Abstract
Black is an important body color of Koi (Cyprinus carpio Koi) which is performed through melanin synthesized by melanocyte, and tyrosinase is an important rate-limiting enzyme in melanin synthesis. It's unclear of genetic mechanism of Koi body color. The present work obtained Koi tyrosinase (Tyr) gene sequence and investigated the expression of Tyr gene in different strains and tissues using semi-quantitative PCR, trying to reveal the relationship between the pigment control genes and the body color. The results showed 1 779 bp of Tyr gene, including a 1 608 bp open reading frame encoding 535 amino acids. Sequence analysis of cDNA and protein and phylogenetic analysis indicated that conservation of Tyr is higher in teleost than between teleost and other vertebrates. Semi-quantitative analysis showed the highest Tyr expression in the eye and black skin, intermediate in brain, red and yellow skin, and the lowest in liver and heart. There was no significant difference of Tyr expression between the same tissues of eyes, liver, heart, brain from different Koi carps, but differentiated in the skins with the highest in black skin, followed by red and yellow skin and small amount in white skin. The different expression was related to tissue function. The role of tyrosinase in non-melanoma cells was unclear. There possibly exist multiple forms of tyrosinase, some of which is inhibited, or the xanthophores and erythrophores could be translated into functional melanocytes.关键词
锦鲤/酪氨酸酶基因/半定量分析/黑色素Key words
Cyprinus carpio Koi/ tyrosinase/ semi-quantitative analysis / melanin分类
生物科学引用本文复制引用
王巍,胡红霞,孙向军,牛翠娟..锦鲤酪氨酸酶基因序列分析及其在不同锦鲤品系不同组织中的表达[J].水产学报,2012,36(11):1658-1666,9.基金项目
北京市重大科技项目(D09060500430901) （D09060500430901）
观赏鱼产业技术体系北京市创新团队建设专项 （）
