四川医学2012，Vol.33Issue(8)：1340-1342,3.
RNA干扰Oct-4增加乳腺癌化疗敏感性的研究
The effect of targeting Oct-4 on bhemosensitivity in breast cancer cells
陈竞 1高砚春 2蔡燕 2王含彦 3侯令密2
作者信息
- 2. 川北医学院附属医院普外三科,检验科,四川南充637000
- 3. 川北医学院生物化学教研室,四川南充637000
- 折叠
摘要
Abstract
Objective To study the effect of targeting Oct-4 on chemosensitivity in breast cancer cells. Methods Small interfering RNA was used to specifically knockdown Oct-4 in breast cancer MCF-7 cells. The mRNA and protein expression of Oct-4 was determined by Real-time PCR and western blot analysis, respectively. MTT assay was completed to detect the percent of cells survival. The apoptosis was determined by Annexin V-F1TC/PI staining and flow cytometry analysis. Results The express-sion of Oct-4 was significantly and specificfy depleted by SiRNA duplexes. After treatment with 0. 02μmol/L doxorubicin for 68 h, ihe percent of cells survival in control( A) , doxorubicin( B) , doxorubicin and negative SiRNA duplexes(C) , and doxorubicin and Oct4 SiRNA duplexes( D) group were 100 ±6. 6% , 46. 7 ±4. 3% , 43. 8 ±4. 6% , and 28. 4 ±3. 5% , respectively. The percent survival in B group was higher than that in D group (P ＜0. 01) . After treatment with 0. 02 μmol/L doxorubicin for 48 h, the apoptosis percent in A, B, C, and D group were 1. 02 ±0. 96% , 18. 81 ±4. 56% , 19. 24 ±4.76% , and 34. 31 ± 5. 14% , respectively. The apoptosis percent in B group was lowerer than that in D group( P ＜0. 01). Conclusion Our result showed that the knochdown of Oct-4 increased the chemosensitivily of doxorubicin against breast cancer cells.关键词
乳腺癌/Oct-4/化疗/肿瘤干细胞Key words
breast cancer/ Oct-4/ chemotherapy, / cancer stem cell分类
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陈竞,高砚春,蔡燕,王含彦,侯令密..RNA干扰Oct-4增加乳腺癌化疗敏感性的研究[J].四川医学,2012,33(8):1340-1342,3.
