山东建筑大学学报2012，Vol.27Issue(5)：465-468,4.
丙酮-超声法提取叶绿素a工艺的优化
Optimization of acetone-ultrasound method to extract the chlorophyll a
摘要
Abstract
The four operating conditions of acetone-ultrasound extraction and spectrophotometric determination of chlorophyll a process ( ultrasonic power, ultrasonic time, rotational speed, centrifugation time) were optimized by orthogonal experiment. The experimental material is chlorella brought up in laboratory to avoid the impact of the algae The results show that the sample sonicated at 225 W power 30min, 6000r/min centrifuged 9min, and that after stirring and repeating ultrasonic and centrifugation step, it can get a better extraction effect.关键词
叶绿素a/提取方法/正交实验法Key words
chlorophyll a/ extraction method/ orthogonal experiment分类
资源环境引用本文复制引用
吴俊森,李冰,潘平..丙酮-超声法提取叶绿素a工艺的优化[J].山东建筑大学学报,2012,27(5):465-468,4.基金项目
国家水体污染控制与治理科技重大专项"引黄供水地区水质风险评估技术研究与应用"(2008ZX07422-001) （2008ZX07422-001）
