山东农业大学学报（自然科学版）2012，Vol.43Issue(4)：508-516,9.
种植年限对牡丹根际土壤微生物群落结构的影响
EFFECTS OF PLANTING YEARS ON MICROBIAL COMMUNITIES' STRUCTURE IN PEONY RHIZOSPHERE SOIL
摘要
Abstract
In order to exploring the effect of peony continuous cropping on the microbial community structure in soil,Samples were collected from peony having been planted for 3 , 5 , 8, 12 and 20 years in Peony Garden of Heze. T - RFLP and DGGE technique was adopted to study the dynamic changes of bacteria and fungi communi ty structure in the rhizosphere soil. The results showed that with increasing year of cropping, the bacteria com munity was not signicantly changed. However the fungi diversity level was significantly reduced, and its commu nity structure tend to be simple. Dice coefficient analysis of DGGE figures showed that bacterial community structure similarity of each peony rhizospheric soil sample were over 76. 9% , but the lowest fungi community structure similarity of each peony rhizospheric soil sample were only 58. 3%. The results from Dice coefficient analysis are similar to that from T - RFLP. The changes of fungi community diversity under continuous cropping of peony might be one of die main reasons causing the obstacles of continuous cropping of peony.关键词
牡丹根际/T-RFLP/DGGE/细菌多样性/真菌多样性Key words
Peony rhizosphere/T-RFLP/DGGE/bacteria diversity/fungi diversity分类
生物科学引用本文复制引用
王雪山,杜秉海,姚良同,王善林,徐峥,丁延芹..种植年限对牡丹根际土壤微生物群落结构的影响[J].山东农业大学学报（自然科学版）,2012,43(4):508-516,9.基金项目
国家科技支撑计划项目(2011BAD11B01) （2011BAD11B01）
