山东农业大学学报（自然科学版）2012，Vol.43Issue(4)：522-528,7.
一株鹅源巴氏杆菌强毒株的分离及鉴定
ISOLATION AND IDENTIFICATION OF A VIRULENT STRAIN FROM GOOSE PASTEURELLA MULTOCIDA
摘要
Abstract
This study was conducted to confirm the pathogen of goose disease in as area of Shandong Province. A strain of goose Pasteurella multocida isola ted and purified from the livers, hearts of the sick breeding geese in an area of Shandong Province based on its morphological and culturd characteristics, biochemical test, PCR and Pathogenicity test and so on. Pathogenicity test showed that the median lethal dose was 10-4.5 CFU/mL of eleven day -old healthy ducklings after being were injected with the ten times diluted bacteria(1. 2 × 109 CFU/mL). The pathological changes of the dead ducklings were similar to that of sick geese, and the strain which had the same morphological characteristics with Pasteurella multocida was reisolated. Histopathological changes were characterized by breaking and of intestinal villus in the duodenum, fatty degeneration in the liver cell and break age of cardiac muscle fibers. Susceptibility test displayed that the strain was sensitive to ceftriaxone, amoxicillin, ampicillin, ofloxacin, and so on. The pathogen of goose disease was Pasteurella multocida anatis isolate in an area of Shandong Province. This provided scientific theory for diagnosing and preventing avian Pasteurella multo cida.关键词
鹅/多杀性巴氏杆菌/分离鉴定/致病性Key words
Goose/Pasteurella multocida/isolation and identification/pathogenicity分类
农业科技引用本文复制引用
宋晓娜,刁有祥,高绪慧,葛平萍,张颖,鞠小军,王丽荣..一株鹅源巴氏杆菌强毒株的分离及鉴定[J].山东农业大学学报（自然科学版）,2012,43(4):522-528,7.基金项目
国家水禽产业技术体系项目(CARS-43-34) （CARS-43-34）
