山东医药2012，Vol.52Issue(34)：22-24,3.
高糖对小鼠足细胞表型转化的诱导作用及机制探讨
Exploration on the mechanism of high glucose-induced epithelial-mesenchymal transition in podocytes
摘要
Abstract
Objective To investigate the mechanism of high glucose-induced epithelial-mesenchymal transition in podocytes. Methods Cultured mouse podocytes were divided into three groups; group A ( cells cultured conventionally) , group B (cells cultured in 30 mmol/L glucose condition) and group C (3 cells cultured in 30 mmol/L glucose and 10 μmol/L AG490 conditions). Cells were collected after cultured 48 h. Western blotting analysis was used to determine the expression of nephrin, α-SMA and p-JAK2. RT-PCR analysis was used to detect the nephrin and α-SMA mRNA. Results The relative absorbance values of nephrin,α-SMA and p-JAK2 were 0.96 ±0.01 ,0.21 ±0.02,0.39 ±0.01 in group A, 0.58 ±0.01,0.66 ±0.07,0.71 ±0.02 in group B, 0.87 ±0.04,0.35 ±0.02,0.41 ±0.04 in group C. The relative absorbance values of nephrin,α-SMA mRNA were 1. 53 ± 0. 04,0. 57 ± 0. 01 in group A ,0. 82 ± 0. 09 ,0. 89 ± 0. 03 in group B,l. 30 ±0.04,0. 78 ±0.03 in group C. Compared with group B, these values in group A and C showed significant difference (all P<0. 05). Conclusion High glucose can induce the epithelial-mesenchymal transition in podocytes by activation of the JAK/STAT pathway.关键词
高糖状态/足细胞/上皮细胞—间充质细胞表型转化/人肾病蛋白/α-平滑肌肌动蛋白/蛋白质酪氨酸激酶/信号通路Key words
high glucose condition/ podocyte/ epithelial-mesenchymal transition/ nephrin/ α smooth mascle actin/ protein tyrosine kinase/ signaling pathway分类
医药卫生引用本文复制引用
刘青娟,张玉军,李建英,付晓辉,姚芳,吴海江,邢玲玲..高糖对小鼠足细胞表型转化的诱导作用及机制探讨[J].山东医药,2012,52(34):22-24,3.基金项目
河北省教育厅科学研究项目(2011168) （2011168）
河北省卫生厅医学科学研究重点课题资项目(20110081). （20110081）
