山东医药2012，Vol.52Issue(39)：24-26,3.
秦皮乙素对人肝癌HepG2细胞体外增殖的影响及机制探讨
Effects of aesculetin on the proliferation of HepG2 in vitro and investigation of its mechanism
摘要
Abstract
Objective To investigate the effects and mechanism of aesculetin on the proliferation of human hepatoma cell HepG2 in vitro. Methods The proliferation effects of different concentrations of aesculetin on HepG2 were observed by MTT assay. The apoptosis and mitochondria] membrance potential were analysed by flow cytomettry (FCM). The activities of Caspase 3, Caspase 8 and Caspase 9 were detected by spectrophotometry. The protein expressions of Bax, Bcl-2 and Caspase 9 were studied by immunofluorescence method. Results Aesculetin inhibited the proliferation of human hepatoma cell HepG2 and induced its apoptosis, and also reduced its mitochondrial membrane potential, enhanced the activities of Caspase 3 and Caspase 9 by reducing mitochondrial membrance in a dose dependent manner; While there was no significant effect on the activity of Caspase 8. Aesculetin promoted Bax protein expression at the same time lowered Bcl-2 protein expression also in a dose dependent manner. Conclusions Aesculetin can inhibit the proliferation of HepG2; the mechanism may induce its apoptosis through mitochondrial pathway in vitor.关键词
秦皮乙素/人肝癌细胞HepG2,凋亡/线粒体途径Key words
aesculetin/ human hepatoma HepG2 cells, the approach of mitochondria/ apoptosis分类
医药卫生引用本文复制引用
伟忠民,王晶..秦皮乙素对人肝癌HepG2细胞体外增殖的影响及机制探讨[J].山东医药,2012,52(39):24-26,3.基金项目
辽宁省教育厅科研项目计划(L2010280). （L2010280）
