上海海洋大学学报2012，Vol.21Issue(6)：945-950,6.
大口黑鲈两个亚种EST数据库分析
Analysis of EST database for two subspecies of largemouth bass
摘要
Abstract
In this study,Roche 454 high-throughput technology was used to conduct transcriptome sequencing and establish ESTs database of the largemouth bass northern subspecies and Florida subspecies.The results showed that a total of 468 671 and 332 322 ESTs were generated from northern subspecies and Florida subspecies.The average length was 306.5 bp and 304.4 bp,respectively.Assembly of the largemouth bass northern subspecies and Florida subspecies' high quality ESTs resulted in 42 056 and 35 743 contigs.The average length was 612.6 bp and 588.2 bp,respectively.EST database of largemouth bass northern subspecies and Florida subspecies was merged and then compared with known protein databases.78 938 EST sequences in total were annotated.By exploring the merged largemouth bass EST library,over 25 469 and 8 547 putative SSRs and SNPs were identified.Comparison of the northern subspecies and Florida subspecies sequence was made by randomly selecting 75 contigs from the EST database.Results showed that EST sequences of the two subspecies homology were 99.2%.关键词
大口黑鲈/北方亚种/佛罗里达亚种/罗氏454测序/表达序列标签Key words
largemouth bass/northern subspecies/florida subspecies/roche 454 sequencing/ESTs分类
农业科技引用本文复制引用
景燕娟,白俊杰,李胜杰,于凌云,蔡磊..大口黑鲈两个亚种EST数据库分析[J].上海海洋大学学报,2012,21(6):945-950,6.基金项目
农业部“948”项目 （）
农业部公益性行业科研专项 （）
国家科技支撑计划项目 （）
