同济大学学报（医学版）2012，Vol.33Issue(5)：41-44,4.DOI:10.3969/j.issn1008-0392.2012.05.009
活性维生素D3对输尿管结扎小鼠肾组织胶原Ⅳ蛋白表达的影响
1,25-dihydroxyvitamin D3ameliorates renal interstitial fibrosis in mice
李瑾 1李微 1殷少军 1陈维杰1
作者信息
- 1. 同济大学附属第十人民医院老年科,上海 200072
- 折叠
摘要
Abstract
Objective To investigate the effect of 1,25-(OH)2VitD3 on renal fibrosis in unilateral ureteral obstruction (UUO) of mice. Methods Forty-two male CD-I mice were randomly assigned to three groups: Sham-operated group (n =6) , UUO group (n = 18) and UUO plus 1,25-(OH)2VitD3 (0.5, 0.25, 1. 0 (xg/kg) group (n = 18). Mice of UUO group were sacrificed 3, 7, 14 d after operation, other groups were sacrificed 7d after operation. The expression of collagen Ⅳ in renal tissues was tested by Western blotting. Results Western blotting demonstrated a significant reduction of collagen IV production in UUO group, especially at d7 following UUO; the contents of collagen Ⅳ in renal tissue of 1, 25-(OH)2VitD3 group were increased compared to UUO group. Conclusion 1, 25-(OH)2VitD3 ameliorates renal interstitial fibrosis in the obstructed kidneys, which is associated with the restoration of collagen Ⅳ production. 关键词
活性维生素D3/胶原Ⅳ/肾间质纤维化Key words
1,25-( OH) 2VitD3/ collagen Ⅳ/ renal interstitial fibrosis分类
医药卫生引用本文复制引用
李瑾,李微,殷少军,陈维杰..活性维生素D3对输尿管结扎小鼠肾组织胶原Ⅳ蛋白表达的影响[J].同济大学学报（医学版）,2012,33(5):41-44,4.
