同济大学学报（医学版）2012，Vol.33Issue(5)：45-48,4.DOI:10.3969/j.issn1008-0392.2012.05.010
小鼠骨髓间充质干细胞的体外培养和鉴定
Culture and identification of mouse bone marrow-derived mesenchymal stem cells
朱建宇 1施伟民2
作者信息
- 1. 上海市皮肤病医院,上海 200435
- 2. 上海交通大学附属第一人民医院皮肤科,上海 200080
- 折叠
摘要
Abstract
Objective To establish an efficient method for culture and isolation of mouse bone marrow-derived mesenchymal stem cells (MSCs). Methods Bone marrow adherent culture was used to isolate and expand MSCs. Inverted microscope was employed to observe the proliferating process and their morphous of primary and passage cells, and immunocyte chemistry was used to identify immunophenotypes of MSCs. Results The MSGs were adherent cells with a similar fibroblastoid spindle-sharp morphology during different passages. The expression rates of CD44, CD34 and CD105 were 94. 3% , 3. 3% and 82. 3%. Conclusion MSCs can be isolated and expanded by using bone marrow adherent culture. 关键词
骨髓间充质干细胞/细胞培养/免疫细胞化学/小鼠Key words
bone marrow mesenchymal stem cells/cell culture/immunocyte chemistry/mouse分类
医药卫生引用本文复制引用
朱建宇,施伟民..小鼠骨髓间充质干细胞的体外培养和鉴定[J].同济大学学报（医学版）,2012,33(5):45-48,4.
