同济大学学报（医学版）2012，Vol.33Issue(5)：49-52,4.DOI:10.3969/j.issn1008-0392.2012.05.011
抗体芯片在类风湿关节炎患者滑液与血浆蛋白检测中的应用
Protein expression profile in synovial fluid and plasma from patients with rheumatoid arthritis using antibody microarrays
程昉 1汤建平 1黄漾 1张晓宇 1房星星1
作者信息
- 1. 同济大学附属同济医院风湿免疫科,上海 200065
- 折叠
摘要
Abstract
Objective To identify differences of protein expression profile between synovial fluid and plasma in patients with rheumatoid arthritis (RA). Methods Paired plasma and synovial fluid samples from 3 patients with RA were analyzed for 507 human proteins by antibody microarrays A standard ＞ 2.0-fold cutoff value was used to determine differentially expressed proteins. Results The array analysis showed that the expression levels of 67 proteins were increased in RA synovial fluid compared with paired plasma. Ten of those proteins were not previously found in synovial fluid or synovium of RA, including IL-28 A, fibroblast growth factor (FGF)-6, FGF-binding protein (FGF-BP), angiopoietin-4, agouti-related protein (AgRP), orexin A, resistin-like molecule (RELM)-(3, nephroblastoma overexpressed (NOV)/ CCN3, growth and differentiation factor-associated serum protein (GASP)-2/WFIKKN, and membrane frizzled-related protein (MFRP). Conclusion This study provides insights into the nature of synovial fluid
protein profile in RA patients and suggests new targets for studying the involvement of proteins in synovitis
and/or bone damage.关键词
关节炎/类风湿/蛋白质组/抗体芯片/滑液Key words
arthritis/ rheumatoid/ proteome/ antibody array/ synovial fluid分类
医药卫生引用本文复制引用
程昉,汤建平,黄漾,张晓宇,房星星..抗体芯片在类风湿关节炎患者滑液与血浆蛋白检测中的应用[J].同济大学学报（医学版）,2012,33(5):49-52,4.
