世界中医药2012，Vol.7Issue(6)：557-559,3.
HPLC法同时测定高冠平胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量
Determination of Notoginsenoside R1,Ginsenoside Rg1,Ginsenoside Re and Ginsenoside Rb1 in Gaoguanping Capsule by HPLC
摘要
Abstract
Objective: To establish an HPLC method for determination of Saponins of Notoginseng Radix et Rhizoma and Ginseng Radix et Rhizoma Rubra in Gaoguanping capsules. Methods:The HPLC was performed on Hibar C18( 250mm ×4. 6mm ), acetonitrile as mobile phase A,water as mobile phase B. The flow rate was 1. 0 mL · min-1. The detection wavelength was 203 nm. Gradient elution: 0-35 min, A: 19% , B: 81% ; 35 ~ 85 min,A:19% →35% ,B: 81% →65% ;85 ~100min, A:35%→19% , B:65→81%. Results: The linear range of notoginsenoside R, , ginsenoside Rg, ginsenoside Re and ginsenoside Rb, was 0.4104 ~ 3.2832μg, 2.0884 ~ 16. 7072μg, 1. 222 ~ 9. 776μg, 2. 0072 ~ 16. 0576μg with good con-elation. The average recovery was 98. 92% , 98. 97% , 99. 01% , 99. 25% , RSD wasl. 08% .,, 1. 06% , 0. 85% , 1. 15%. Conclusion: The method is simple, accurate and reproduciable. It can be used for quality control of notoginsenoside R, , ginsenoside Rg, , ginsenoside Re and ginsenoside Rb, in Gaoguanping capsules.关键词
高效液相色谱法/高冠平胶囊/三七皂苷R1/人参皂苷Rg1/人参皂苷Re/人参皂苷Rb1Key words
HPLC/ Gaoguanping capsules/ Notoginsenoside R1 / Ginsenoside Rg1 / Ginsenoside Re/ Ginsenoside Rb1引用本文复制引用
苏英英,宋敏,杨东花,刘峰..HPLC法同时测定高冠平胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量[J].世界中医药,2012,7(6):557-559,3.基金项目
2009年度陕西省科学技术研究发展计划项目(编号:2009k19-03) （编号:2009k19-03）
