生命科学研究2012,Vol.16Issue(5):377-381,5.
非生物胁迫对花生叶片AhNCED1蛋白表达的影响
Effect of Abiotic Stresses on AhNCED1 Expression of Peanut Leaves
摘要
Abstract
The 9-cw-epoxycarotenoid dioxygenasel in Arachis hypogaea L. (AhNCEDl) is thought to be the rate-limiting enzyme in the abscisic acid (ABA) biosynthetic pathway in peanut. The effect of abiotic stresses on AhNCEDl expression and ABA content of peanut leaves were studied. The AhNCEDl expression in leaves were enhanced rapidly at 1 h under osmotic stress, and the ABA content were increased along with the stress time. The AhNCEDl expression were enhanced after treat with NaCl for 7 h, and the ABA content were increased significantly after stress for 10 h. After low temperature treatment for 10 h, AhNCEDl expression were enhanced, and then ABA content were also increased. There was almost no effect of high temperature on AhNCEDl expression in peanut leaves, and the ABA content were not changed nearly. These results showed that the peanut may through activating AhNCEDl expression to enhance the ABA level in response to abiotic stresses, to get used to outside ambient environment.关键词
非生物胁迫/花生/AhNCED1/蛋白表达/ABA含量Key words
abiotic stresses/peanut/9-ew-epoxycarotenoid dioxygenasel in Arachis hypogaea L. (AhNCEDl)/protein expression/ABA content分类
生物科学引用本文复制引用
胡博,刘帅,李玲..非生物胁迫对花生叶片AhNCED1蛋白表达的影响[J].生命科学研究,2012,16(5):377-381,5.基金项目
国家自然科学基金资助项目(30971715) (30971715)
中国博士后科学基金资助项目(20110490907) (20110490907)
广东省科技计划资助项目(2011B020301009) (2011B020301009)