色谱2012，Vol.30Issue(9)：883-888,6.DOI:10.3724/SP.J.1123.2012.05023
高效液相色谱-串联质谱法检测鸡肉中9种化学合成类抗球虫药多残留
Simultaneous extraction and determination of nine chemically synthetic anticoccidial drug multi-residues in chicken meat using high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/ MS) method was developed for the simultaneous extraction and determination of multi-residues of nine chemically synthetic anticoccidial drugs in chicken meat. Toltrazuril-D3 was used as the internal standard. The sample was extracted by 15 mL acetonitrile/dimethyl sulfoxide (4:1, v/ v, containing 2% acetic acid), cleaned-up by a QuEChERS (quick, easy, cheap, effective, rugged and safe) clean-up tube with 150 mg octadecylsilyl (ODS), 100 mg Florisil and 100 mg graphitized carbon black (GCB). After the concentration, the extract was analyzed by HPLC-MS/MS. The results showed that good linearity in the range of 20-150 jig/L for each target compound. The recoveries were between 81.5% and 103.6% with the relative standard deviations lower than 15%. The method is simple, rapid, and suitable to detect the chemically synthetic anticoccidial drug residues with high throughput.关键词
高效液相色谱-串联质谱/化学合成抗球虫药/多残留/鸡肉Key words
high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)/ chemically synthetic anticoccidial drugs/ multi-residues/ chicken meat分类
化学化工引用本文复制引用
施祖灏,张小燕,卜士金,陈大伟,蒲俊华,高玉时,唐修君..高效液相色谱-串联质谱法检测鸡肉中9种化学合成类抗球虫药多残留[J].色谱,2012,30(9):883-888,6.基金项目
国家科技支撑计划项目(2012BAD39B04-3)、江苏省自然科学基金项目(BK2010299)和扬州市社会发展项目(2008040). （2012BAD39B04-3）
