色谱2012，Vol.30Issue(9)：908-914,7.DOI:10.3724/SP.J.1123.2012.06019
超高效液相色谱-电喷雾串联四极杆质谱法同时测定配合饲料中29种兽药
Simultaneous determination of 29 veterinary drugs in compound feeds by ultra performance liquid chromatography-electrospray ionization tandem quadrupole mass spectrometry
摘要
Abstract
An ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed for the simultaneous determination of 29 veterinary drugs of quinolones, sulfonamides, macrolides and nitrofurans in compound feeds. The sample was extracted with methanol-acetonitrile (1=1, v/v), and then cleaned up using solid phase extraction with an Oasis HLB column, detected by UPLC-ESI-MS/ MS. The UPLC separation was performed on a C,, column (100 mm x2.1 mm, 1.7 |xm) using a gradient elution with the mobile phases of methanol and 0.1% (v/v) formic acid aqueous solution. The ESI-MS/MS detection was achieved in positive mode under multiple reaction monitoring (MRM) mode. The linear ranges were from 0.01 to 5.0 mg/L with the correlation coefficients above 0.99 for all the 29 veterinary drugs. The average recoveries of the 29 veterinary drugs spiked in three feed matrixes (the compound premix feed, the complete formula feed and the concentrated feed) at four spiked levels were 61.2% - 94.3% with the relative standard deviations (RSDs) of 2.2% -15.0%. The limits of detection were 0.01 mg/kg or 0. OS mg/kg. The method is rapid, accurate, reproducible, sensitive and easy to apply, and suitablefor the simultaneous determination of diverse veterinary drugs in compound feeds.关键词
超高效液相色谱-电喷雾串联四极杆质谱/兽约/配合饲料Key words
ultra performance liquid chromatography-electrospray ionization tandem quadru-pole mass spectrometry (UPLC-ESI-MS/MS) / veterinary drugs/ compound feeds分类
化学化工引用本文复制引用
赵颖,刘瑜,金雁,徐宜宏,钟钰,蒋施,李晓东,曾凡,周健南..超高效液相色谱-电喷雾串联四极杆质谱法同时测定配合饲料中29种兽药[J].色谱,2012,30(9):908-914,7.基金项目
辽宁检验检疫局科研项目(LK21-2009). （LK21-2009）
