色谱2012，Vol.30Issue(9)：957-961,5.DOI:10.3724/SP.J.1123.2012.04026
超高效液相色谱-串联质谱法测定果汁中的展青霉素
Determination of patulin in juice by ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method was developed for the simultaneous determination of patulin in juice by solid phase extraction (SPE) -ultra performance liquid chromatography coupled with electro-spray ionization tandem, mass spectrometry (UPLC-ESI-MS/MS). The cloudy juice was hydro-lyzed by enzyme and extracted by ethyl acetate. The extract of cloudy juice was then enriched and purified by an HLB SPE cartridge. If the juice was clear it was directly treated by the cartridge as above. The separation was performed on a C18 column with the gradient elution of acetonitrile and water. The patulin was detected by MS with electrospray negative ionization (ESI' ) in the mode of multiple reaction monitoring (MRM). The good linearity was observed in the range of 1.0 - 500 jig/L with the correlation coefficient of 0.999 and the limit of quantita-tion of 5.0 jig/kg. The recoveries were in the range of 80.6% -91. 8% at three spiked levels of 5.0, 25. 0 and 100.0 ng/kg with the relative standard deviations of 1.5% -7.3%. It was proved to be a simple, reliable and accurate method which can fully meet the requirements of patulin detection in juice samples according to most domestic and international legislations.关键词
超高效液相色谱-串联质谱/固相萃取/展青霉素/果汁Key words
ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)/ solid phase extraction (SPE)/ patulin/ juice分类
化学化工引用本文复制引用
牛华,冯雷,牛之瑞,珠娜,祝红昆,王亚琴..超高效液相色谱-串联质谱法测定果汁中的展青霉素[J].色谱,2012,30(9):957-961,5.基金项目
云南省食品安全检测科技服务平台(2009DA022)和云南省食品安全地方标准项目. （2009DA022）
