水生生物学报2012，Vol.36Issue(6)：1056-1062,7.DOI:10.3724/SP.J.1035.2012.01056
急性高渗胁迫对中华绒螯蟹雄蟹组织中可溶性蛋白质、血蓝蛋白、血糖与肝糖原含量的影响
EFFECT OF ACUTE SALINITY STRESS ON SOLUBLE PROTEIN, HEMOCYANIN, HAEMOLYMPH GLUCOSE AND HEPATOPANCREAS GLYCOGEN OF ERIOCHEIR SINENSIS
摘要
Abstract
Most of the decapod species of crustacean have comprehensive capacity of osmoregulation, and they can adapt to relative wide ambient salinity range. It is important to understand the underlying mechanisms of their comprehensive adaptability to salinity change because this knowledge is helpful for inland culturing of marine species. As a euryhaline crustacean, the Chinese mitten crab, Eriocheir sinensis, has been well studied in many aspects related to their physiological responses to salinity change, and many useful literature findings have been accumulated. Therefore, E. sinensis can be considered as an animal model for studying osmoregulation mechanism of the decapod species. However, the roles of nutrients in osmoregulation of E. sinensis are still limited. In this paper, male E. sinensis in freshwater (0.3%o) were transferred directly into waters at salinity values of 16%o and 30%o, respectively, and the crabs kept at fresh water served as the control. Each treatment has three replicates. The soluble protein in haemolymph and hepatopancreas, hemocyanin, haemo-lymph glucose and hepatopancreas glycogen at Oh, 6h, 12h, 24h, 48h, 72h and 96h of E. sinensis at different salinities were determined. Soluble protein of haemolymph and hepatopancreas were measured with a Folin reagent based on a standard curve using bovine serum albumin as the standard protein. Hemocyanin was assayed using an ultraviolet absorption methods at 335 nm and calculated according to Nickerson =2.83. Haemolymph glucose was assayed using glucose oxi- dase-peroxide enzyme (GOD-POD) method. Hepatopancreas glycogen was measured using the anthrone colorimetry method. The results showed that the crabs at salinity 16%o and 30%o had significantly lower soluble protein content of hepatopancreas than those at fresh water (P<0.05) when crabs had encountered salt water for 12h?6h, and the soluble protein content of haemolymph decreased significantly from 6h to 48h, and then increased at 72h. The hemocyanin content showed a significantly decreasing trend from Oh to 24h, and then it increased after 48h. Hepatopancreas glycogen contents at salinity 16%o and 30%o were significantly lower than those at fresh water (P<0.05) from 6h to 96h, while no significant differences were observed between crabs in waters with salinity 16%o and 30%o (P>0.05). Haemolymph glucose content at 16%o reduced significantly (TO.05) from 24h to 48h, and gradually recovered up at 72h, but it showed a significantly decreasing trend from 6h to 12h at salinity 30%o, and then gradually increased after 24h. All these results indicated that E. sinensis could produce physiological and biochemical adaptation to maintain a stable osmotic pressure during acute salinity stress. Carbohydrate and protein play important roles in the osmoregulation of crustaceans. E. sinensis could use carbohydrate first to provide energy under acute salinity stress. The higher the salinity, the faster the haemolymph glucose was consumed, and it also recovered more quickly. This observation suggested that carbohydrate could be a direct source in osmoregulation. The crabs may use protein to maintain the balance of osmotic pressure under high salinity by metabolizing the protein into free amino acids. Beside providing free amino acids, hemocyanin also can carry oxygen to meet the needs for physiological activities. Therefore, more attention should be paid to carbohydrate and protein supplied under salinity stress. The ratio of free amino acids maintaining the balance of osmotic pressure and supplying energy by oxidation and the utilization of lipid in osmoregulation require further study. Since the lipid contents in haemolymph and hepatopancreas were not determined in this study, the role of body lipid in E. sinensis was not discussed here and should be further examined.关键词
盐度/中华绒螯蟹/可溶性蛋白/血蓝蛋白/肝糖原/血糖Key words
Salinity/ Eriocheir sinensis/ Soluble protein/ Hemocyanin/ Hepatopancreas glycogen/ Haemolymph glucose分类
生物科学引用本文复制引用
王悦如,李二超,陈立侨,王晓丹,张凤英,高露姣,龙丽娜..急性高渗胁迫对中华绒螯蟹雄蟹组织中可溶性蛋白质、血蓝蛋白、血糖与肝糖原含量的影响[J].水生生物学报,2012,36(6):1056-1062,7.基金项目
公益性行业(农业)科研专项(201003020 （农业）
201203065) （）
“十二五”国家科技支撑计划课题(2012BAD25B00) （2012BAD25B00）
国家自然科学基金项目(31172422、31001098) （31172422、31001098）
上海市中华绒螯蟹产业技术体系建设 （）
上海市科技兴农推广项目(沪农科推字2009-2-1) （沪农科推字2009-2-1）
上海市科委重点项目(10JC1404100) （10JC1404100）
上海市自然科学基金(09ZR1409800) （09ZR1409800）
高等学校博士点专项基金(20100076120006) （20100076120006）
农业部海洋与河口渔业资源及生态重点开放实验室基金部分资助 （）
