水生生物学报2012，Vol.36Issue(6)：1156-1163,8.DOI:10.3724/SP.J.1035.2012.01156
大黄鱼微卫星标记的开发及其遗传方式分析
ISOLATION AND GENETIC ANALYSIS OF MICROSATELLITE MARKERS FOR LARIMICHTHYS CROCEA
摘要
Abstract
We constructed an (AC)n-microsatellite-enriched library for large yellow croaker Larimichthys crocea (Richardson 1846) using the method of FIASCO. Ninety clones were randomly selected for further colony amplification. The rate of positive clones reached to 66.7%, and 56 of the 60 positive clones sequences contained one or more SSR. By the analysis of repeat motif, dinucleotide repeats were the most dominant (91.1%), followed by trinucleotide repeats (8.9%). Among dinucleotides, (AC)n repeats were the most frequent (94.1%). Using Weber's classification rules, the sequences were divided into three categories, the percentage of perfect repeat sequences, imperfect repeat sequences, and compound repeat sequences was 75.0%, 8.9%, and 16.1, respectively. Fifty two microsatellites primer pairs were designed and synthesized. The result showed that 35 SSRs were polymorphic in 46 Fl generation individuals. Twenty eight (80.0%) loci exhibited a segregation ratio of 1 '. 1 '. 1 '. 1 (AB x CD/AB x AC), serving as the most useful markers segregating in co-dominant fashion. Six loci exhibited a segregation ratio of 1 '. 1, and one loci exhibited a segregation of 1 '. 2 '. 1. Polymorphism analysis showed that 32 SSRs were consisted with Mendelian segregation ratio and could be used to construct the linkage map, while the other 3 SSRs (LZC0137, LZC0139, LZC0152) were departure from the expected Mendelian segregation patterns. These SSRs should be available for genetic linkage mapping, population genetics studies, molecular evolution and phylogenetic study of large yellow croaker.关键词
大黄鱼/微卫星/孟德尔分离Key words
Larimichthys crocea/ Micro satellite-enriched library/ Mendelian segregation patterns分类
生物科学引用本文复制引用
叶华,任鹏,刘洋,刘贤德,王志勇..大黄鱼微卫星标记的开发及其遗传方式分析[J].水生生物学报,2012,36(6):1156-1163,8.基金项目
国家自然科学基金(30771663) （30771663）
国家公益性行业(农业)科研专项(200903029-04) （农业）
集美大学创新团队科研基金(2011A001) 资助 （2011A001）
