生态学杂志2012，Vol.31Issue(10)：2479-2485,7.
抗草甘膦转基因大豆对根际土壤氨氧化古菌群落多样性的影响
Effects of roundup ready transgenic soybean on ammonia-oxidizing archaeal diversity in rhizospheric soil
摘要
Abstract
Roundup ready transgenic soybean [ Glycine max (L. ) Merr. ] (RRS) is one of the genetically modified crops and firstly commercialized by the Monsanto Company. In recent years, more attention has been paid on the risk assessment of RRS, including the potential effects on soil biota diversity. Ammonia-oxidizing archaea (AOA) are a group of the key microbes in nitrification, playing an important role in N cycle. By using PCR-DGGE cloning, this paper studied the diversity of AOA communities in the rhizosphere soils of RRS, its non-transgenic parental iso-line (RRS-S) , wild soybean (W-S) , and cultivated soybean (D-46) , aimed to provide theoretical basis for the ecological safety assessment on the transgenic soybean. The RRS had no remarkable effects on the AOA diversity, based on the Shannon diversity index and evenness index. The principal components analysis and DGGE analysis showed that the AOA community structure in the rhizosphere of RRS was significantly different with that in the rhizosphere of WS and D-46, but had no significant difference with that in the rhizosphere of RRS-S. Moreover, the AOA amok gene sequencing of DGGE bands and the phylogenetic analysis revealed that parts of the AOA in the rhizosphere of test soybean cultivars belonged to the clusters already reported in soil/ sediment, others belonged to the unknown clusters or groups in soil, but none of them belonged to the clusters in water or sediment. There was little change in the DGGE bands, among which,bands 1, 20, and 25 were missing, and band 3 was specific for RRS. In conclusion, RRS had no apparent effect on the diversity of AOA in rhizosphere soils, but could alter the composition of AOA community.关键词
风险评价/PCR-DGGE/多样性/amoAKey words
risk assessment/ PCR-DGGE/ diversity/ amoA.分类
农业科技引用本文复制引用
刘志华,徐广惠,王宏燕,刘佳..抗草甘膦转基因大豆对根际土壤氨氧化古菌群落多样性的影响[J].生态学杂志,2012,31(10):2479-2485,7.基金项目
转基因生物新品种重大培育项目(2009ZX08011-025B)和黑龙江省自然科学基金项目(04-0402:ZJN)资助. （2009ZX08011-025B）
